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Effect of dietary supplementation with carotenoids on xenobiotic metabolizing enzymes in the liver, lung, kidney and small intestine of the rat.
- Source :
-
The British journal of nutrition [Br J Nutr] 1999 Mar; Vol. 81 (3), pp. 235-42. - Publication Year :
- 1999
-
Abstract
- The effect of 16 d intake of 300 mg carotenoids/kg diet (beta-carotene (beta C), bixin (BX), lycopene (LY), lutein (LU), canthaxanthin (CX) or astaxanthin (AX) on xenobiotic metabolizing enzymes in the liver, lung, kidney and small intestine of male Wistar rats was assessed. A control group received the basal diet (AIN-76) without carotenoids and a positive control group for enzyme induction received 3-methylcholanthrene (3-MC) at 666 mg/kg diet. Cytochrome P450 activity was assessed using the substrates ethoxyresorufin for P450 1A1, methoxyresorufin for P450 1A2, pentoxyresorufin for P450 2B1/2 and benzyloxyresorufin for P450 types 1A1/2, 2B1/2 and 3A. Glutathione-S-transferase (EC 2.5.1.18) and reduced glutathione status were assessed. Carotenoid uptake by the tissues was also determined. 3-MC and the carotenoids BX, CX and AX led to significant increases compared with control in liver, lung and kidney ethoxyresorufin-O-deethylation. Methoxyresorufin-O-demethylation activity was significantly increased in liver and lung by BX, CX and AX but only CX and AX significantly increased activity in kidney. Pentoxyresorufin-O-depentylation and benzyloxyresorufin-O-dearylation increased in liver of 3-MC-, BX-, CX- and AX-treated rats, but to a much lesser degree than for the other two substrates. Benzyloxyresorufin-O-dearylation in lung was significantly decreased by all carotenoids. Activities of any of the measured enzymes in the small intestine were undetectable in all treatment groups except the 3-MC group. Glutathione status was unaffected by any of the treatments. This is the first study identifying the carotenoids BX, CX and AX as inducers of rat lung and kidney xenobiotic metabolizing enzymes.
- Subjects :
- Animals
Anticarcinogenic Agents administration & dosage
Anticarcinogenic Agents metabolism
Canthaxanthin administration & dosage
Canthaxanthin metabolism
Carcinogens administration & dosage
Carcinogens metabolism
Carotenoids chemistry
Carotenoids metabolism
Enzyme Activation
Glutathione metabolism
Glutathione Transferase metabolism
Intestine, Small enzymology
Lutein administration & dosage
Lutein chemistry
Lutein metabolism
Lycopene
Male
Methylcholanthrene administration & dosage
Methylcholanthrene metabolism
Rats
Rats, Wistar
Xanthophylls
Xenobiotics administration & dosage
beta Carotene administration & dosage
beta Carotene analogs & derivatives
beta Carotene metabolism
Carotenoids administration & dosage
Cytochrome P-450 Enzyme System metabolism
Kidney enzymology
Liver enzymology
Lung enzymology
Xenobiotics metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0007-1145
- Volume :
- 81
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The British journal of nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 10434850