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Coupling of mGluR/Homer and PSD-95 complexes by the Shank family of postsynaptic density proteins.
- Source :
-
Neuron [Neuron] 1999 Jul; Vol. 23 (3), pp. 583-92. - Publication Year :
- 1999
-
Abstract
- Shank is a recently described family of postsynaptic proteins that function as part of the NMDA receptor-associated PSD-95 complex (Naisbitt et al., 1999 [this issue of Neuron]). Here, we report that Shank proteins also bind to Homer. Homer proteins form multivalent complexes that bind proline-rich motifs in group 1 metabotropic glutamate receptors and inositol trisphosphate receptors, thereby coupling these receptors in a signaling complex. A single Homer-binding site is identified in Shank, and Shank and Homer coimmunoprecipitate from brain and colocalize at postsynaptic densities. Moreover, Shank clusters mGluR5 in heterologous cells in the presence of Homer and mediates the coclustering of Homer with PSD-95/GKAP. Thus, Shank may cross-link Homer and PSD-95 complexes in the PSD and play a role in the signaling mechanisms of both mGluRs and NMDA receptors.
- Subjects :
- Animals
Binding Sites physiology
COS Cells
Calcium metabolism
Calcium Channels metabolism
Carrier Proteins chemistry
Carrier Proteins genetics
Disks Large Homolog 4 Protein
Homer Scaffolding Proteins
Humans
Inositol 1,4,5-Trisphosphate Receptors
Intracellular Signaling Peptides and Proteins
Kidney cytology
Membrane Proteins
Microscopy, Immunoelectron
Mutagenesis, Site-Directed physiology
Neurons metabolism
Neuropeptides chemistry
Proline metabolism
Protein Structure, Tertiary
Rabbits
Rats
Receptors, Cytoplasmic and Nuclear metabolism
Receptors, N-Methyl-D-Aspartate metabolism
SAP90-PSD95 Associated Proteins
Synapses chemistry
Synapses metabolism
Synapses ultrastructure
Transfection
Adaptor Proteins, Signal Transducing
Carrier Proteins metabolism
Nerve Tissue Proteins metabolism
Neurons chemistry
Neuropeptides metabolism
Receptors, Metabotropic Glutamate metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0896-6273
- Volume :
- 23
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Neuron
- Publication Type :
- Academic Journal
- Accession number :
- 10433269
- Full Text :
- https://doi.org/10.1016/s0896-6273(00)80810-7