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Quantification of the flavonoid-degrading bacterium Eubacterium ramulus in human fecal samples with a species-specific oligonucleotide hybridization probe.
- Source :
-
Applied and environmental microbiology [Appl Environ Microbiol] 1999 Aug; Vol. 65 (8), pp. 3705-9. - Publication Year :
- 1999
-
Abstract
- To investigate the occurrence of the flavonoid-degrading bacterium Eubacterium ramulus in the human intestinal tract, an oligonucleotide probe designated S-S-E.ram-0997-a-A-18 was designed and validated, with over 90 bacterial strains representing the dominant described human fecal flora. Application of S-S-E. ram-0997-a-A-18 to fecal samples from 20 subjects indicated the presence of E. ramulus in each individual tested in numbers from 4.4 x 10(7) to 2.0 x 10(9) cells/g of fecal dry mass. Six fecal E. ramulus isolates were recognized by S-S-E.ram-0997-a-A-18 but exhibited different band patterns when analyzed by randomly amplified polymorphic DNA.
Details
- Language :
- English
- ISSN :
- 0099-2240
- Volume :
- 65
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Applied and environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 10427069
- Full Text :
- https://doi.org/10.1128/AEM.65.8.3705-3709.1999