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Histological analysis and ancient DNA amplification of human bone remains found in caius iulius polybius house in pompeii.
- Source :
-
Croatian medical journal [Croat Med J] 1999 Sep; Vol. 40 (3), pp. 392-7. - Publication Year :
- 1999
-
Abstract
- Thirteen skeletons found in the Caius Iulius Polybius house, which has been the object of intensive study since its discovery in Pompeii 250 years ago, have provided an opportunity to study either bone diagenesis by histological investigation or ancient DNA by polymerase chain reaction analysis. DNA analysis was done by amplifying both X- and Y-chromosomes amelogenin loci and Y-specific alphoid repeat locus. The von Willebrand factor (vWF) microsatellite locus on chromosome 12 was also analyzed for personal identification in two individuals showing alleles with 10/11 and 12/12 TCTA repeats, respectively. Technical problems were the scarcity of DNA content from osteocytes, DNA molecule fragmentation, microbial contamination which change bone structure, contaminating human DNA which results from mishandling, and frequent presence of Taq DNA polymerase inhibiting molecules like polyphenols and heavy metals. The results suggest that the remains contain endogenous human DNA that can be amplified and analyzed. The amplifiability of DNA corresponds to the bone preservation and dynamics of the burial conditions subsequent to the 79 A.D. eruption.
- Subjects :
- Alleles
Amelogenin
Chromosome Mapping
Chromosomes, Human, Pair 12 genetics
DNA genetics
DNA Fragmentation
Dental Enamel Proteins genetics
Enzyme Inhibitors adverse effects
Female
Gene Amplification
History, Ancient
Humans
Italy
Male
Metals adverse effects
Microsatellite Repeats genetics
Osteocytes metabolism
Phenols adverse effects
Polymerase Chain Reaction
Polymers adverse effects
Polyphenols
Repetitive Sequences, Nucleic Acid genetics
Taq Polymerase antagonists & inhibitors
X Chromosome genetics
Y Chromosome genetics
von Willebrand Factor genetics
Bone and Bones anatomy & histology
DNA analysis
Flavonoids
Paleontology
Subjects
Details
- Language :
- English
- ISSN :
- 0353-9504
- Volume :
- 40
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Croatian medical journal
- Publication Type :
- Academic Journal
- Accession number :
- 10411967