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Trk-neurotrophin receptor-like immunoreactivity in the gut of teleost species.
- Source :
-
Cell and tissue research [Cell Tissue Res] 1999 May; Vol. 296 (2), pp. 323-30. - Publication Year :
- 1999
-
Abstract
- Neurotrophins, acting through their high-affinity signal-transducing Trk receptors, are involved in the development, differentiation and maintenance of discrete neuron populations in the higher vertebrates. Furthermore, the presence of Trk receptors in some non-neuronal tissues, including the endocrine cells of the gut, could indicate an involvement of neurotrophins also in these tissues. Recently, neurotrophins and neurotrophin receptor proteins have been identified in the lower vertebrates and invertebrates, whose amino acid sequences are highly homologous with those found in mammals. The present study investigates the occurrence and distribution of Trk-like proteins in the neurons and gut endocrine cells in five species of teleost. Single and double immunolabeling was carried out on fresh and paraffin-embedded tissue using commercially available antibodies against sequences of the intracytoplasmic domain of the mammalian Trk. Western-blot analysis, carried out on samples of stomach and intestine of bass, identified proteins whose estimated molecular masses (140 kDa, 145 kDa and 143-145 kDa) were similar to those reported for full-length TrkA, TrkB and TrkC in the higher vertebrates. TrkA-like immunoreactivity was found in the enteric nervous system plexuses of three fish species. Trk-like immunoreactivity was observed in the endocrine cells as follows: sparse TrkA-like immunoreactive endocrine cells were detected only in the intestine: TrkB-like immunoreactive cells were detected only in the stomach; and TrkC-like immunoreactive cells were found both in the intestine of the carp and in the stomach of the bass, where they also showed TrkB-like immunoreactivity. These findings confirm the occurrence and distribution of Trk-like proteins in teleosts. These proteins are closely related to the Trk neurotrophin receptors of mammals. The functional significance of Trk-like proteins in both neuronal and non-neuronal cells of teleosts is still not clear.
- Subjects :
- Animals
Bass
Carps
Goldfish
Mammals
Perciformes
Receptor, Ciliary Neurotrophic Factor
Receptor, trkC
Species Specificity
Fishes
Gastric Mucosa innervation
Intestinal Mucosa innervation
Intestines innervation
Myenteric Plexus cytology
Neurons cytology
Receptor Protein-Tyrosine Kinases analysis
Receptor, trkA analysis
Receptors, Nerve Growth Factor analysis
Stomach innervation
Subjects
Details
- Language :
- English
- ISSN :
- 0302-766X
- Volume :
- 296
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Cell and tissue research
- Publication Type :
- Academic Journal
- Accession number :
- 10408916
- Full Text :
- https://doi.org/10.1007/s004410051292