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Cytochrome c is dispensable for fas-induced caspase activation and apoptosis.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1999 Jul 22; Vol. 261 (1), pp. 71-8. - Publication Year :
- 1999
-
Abstract
- Cytochrome c is thought to play an important role in the initiation of apoptosis following its release from mitochondria. It is controversial whether such release is also involved in caspase activation and apoptotic cell death after ligation of the cell surface molecule Fas. We addressed this issue by investigating cells from the human cell lines Jurkat and SKW6 which had been treated with the inhibitor of the mitochondrial F0/F1-ATPase, oligomycin. Oligomycin-treatment led, over a wide range of concentrations, to ATP-depletion and, at similar concentrations, abrogated the appearance of caspase-3-like activity caused by stauroporine. Electroporation of cytochrome c protein into intact cells induced caspase activation in both cell lines and significant nuclear apoptosis in Jurkat cells. In ATP-depleted cells, electroporation of cytochrome c induced neither caspase activation nor nuclear fragmentation. Fas-induced caspase activation and nuclear apoptosis, however, were unaffected by the depletion of ATP. Thus, cytochrome c is unlikely to be an important factor in Fas-induced cell death.<br /> (Copyright 1999 Academic Press.)
- Subjects :
- Adenosine Triphosphate metabolism
Coumarins metabolism
Cytochrome c Group administration & dosage
Dose-Response Relationship, Drug
Electroporation
Enzyme Activation drug effects
Humans
Jurkat Cells
Oligomycins pharmacology
Oligopeptides metabolism
Proton-Translocating ATPases antagonists & inhibitors
Signal Transduction drug effects
Staurosporine antagonists & inhibitors
Staurosporine pharmacology
Tumor Cells, Cultured
Apoptosis drug effects
Caspases metabolism
Cytochrome c Group physiology
fas Receptor physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 261
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 10405325
- Full Text :
- https://doi.org/10.1006/bbrc.1999.0942