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Enhanced expression of HLA class I molecules in human hepatocellular carcinoma cells transduced with gamma-interferon gene.
- Source :
-
Chinese medical journal [Chin Med J (Engl)] 1998 Apr; Vol. 111 (4), pp. 319-22. - Publication Year :
- 1998
-
Abstract
- Objective: To investigate the expression of exogenous gamma-interferon gene in human hepatocellular carcinoma cells following retroviral transduction and the effect on the expression of surface HLA class I molecules.<br />Methods: Retroviral vector pLXSN was used to introduce human gamma-interferon (IFN-gamma) gene into four different human hepatocellular carcinoma cell lines (HCC). The G418-resistant colonies were isolated and cloned. The integration and expression of IFN-gamma gene were determined by PCR and RT-PCR analysis. A bioassay method was used to test the amount of IFN-gamma secreted by gene modified HCC cells. The expression of HLA class I molecules in HCC cells were analyzed by flow cytometry using indirect fluorescence staining.<br />Results: Four different HCC cell lines were successfully transduced with human IFN-gamma gene using retroviral vector. The integration and expression of IFN-gamma gene were shown only in the transduced cells. All four genetically modified HCC cells can secrete varied amount of IFN-gamma and demonstrate a significant up-regulation of surface HLA class I antigens. One specific HLA class I antigen, HLA-A2, has almost the same degree of increase as that of the total HLA class I molecules after transduction with IFN-gamma gene.<br />Conclusions: Gene modification with IFN-gamma gene can significantly enhance the expression of HLA class I molecules in HCC cells and may increase its immunogenicity. These gene modified tumor vaccines can be helpful in tumor biotherapy.
- Subjects :
- Gene Transfer Techniques
Genes, MHC Class I genetics
HLA-A1 Antigen immunology
HLA-A2 Antigen biosynthesis
HLA-A2 Antigen immunology
Humans
Transduction, Genetic
Tumor Cells, Cultured
Carcinoma, Hepatocellular genetics
HLA-A1 Antigen biosynthesis
Interferon-gamma genetics
Liver Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0366-6999
- Volume :
- 111
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Chinese medical journal
- Publication Type :
- Academic Journal
- Accession number :
- 10374395