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Lack of natural killer cell precursors in fetal liver of Ikaros knockout mutant mice.
- Source :
-
Natural immunity [Nat Immun] 1998; Vol. 16 (4), pp. 137-45. - Publication Year :
- 1998
-
Abstract
- The role of Ikaros in early stages of natural killer (NK) cell differentiation was investigated using an in vitro system that promotes proliferation and differentiation of NK cell precursors into mature NK1.1+ cells. Day 14.5 fetal liver cells from mice, either homozygous for Ikaros Null or dominant negative (DN) mutations, had severe 55- to 79-fold reductions in functional NK cell precursors. Although there was no statistically significant difference between values for +/+ and +/- Null mice, the mean precursor frequency for DN mutant (+/-) mice was significantly above that for DN -/- mice and below that for DN +/+ mice. The NK activity values for cells generated from the NK cell precursors followed the same respective relationships found for NK cell precursor frequencies. These data suggest that the deficiency of mature NK cells in Ikaros mutant mice is related to lack of functional precursors.
- Subjects :
- Animals
Female
Fetus cytology
Heterozygote
Homozygote
Ikaros Transcription Factor
In Vitro Techniques
Liver cytology
Mice
Mice, Knockout
Pregnancy
Transcription Factors deficiency
DNA-Binding Proteins
Fetus immunology
Hematopoietic Stem Cells immunology
Killer Cells, Natural immunology
Liver immunology
Transcription Factors genetics
Transcription Factors immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1018-8916
- Volume :
- 16
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Natural immunity
- Publication Type :
- Academic Journal
- Accession number :
- 10366784
- Full Text :
- https://doi.org/10.1159/000069438