Back to Search
Start Over
Role of the cytoplasmic tail of gE in antibody-induced redistribution of viral glycoproteins expressed on pseudorabies-virus-infected cells.
- Source :
-
Virology [Virology] 1999 Jun 20; Vol. 259 (1), pp. 141-7. - Publication Year :
- 1999
-
Abstract
- Pseudorabies virus (PrV) glycoprotein gE is a nonessential glycoprotein involved in virulence and spread of the virus. It also has an important, yet unknown, function during antibody-induced capping of viral glycoproteins on the plasma membrane of PrV-infected swine kidney cells. In the present study, it was shown, by the use of a PrV strain expressing a truncated gE glycoprotein, that the cytoplasmic tail of gE is of significant importance for viral glycoprotein capping to occur. In addition, using PrV strains carrying point mutations in the cytoplasmic tail of gE, it was demonstrated that two tyrosine-based motifs are very important for correct functioning of gE during viral glycoprotein capping. Furthermore it was shown that genistein and tyrphostin, two tyrosine kinase activity inhibitors, inhibit viral glycoprotein capping in a concentration-dependent manner. In conclusion, it can be stated that efficient antibody-induced viral glycoprotein capping requires the presence of two YxxL sequences in the cytoplasmic tail of glycoprotein gE, as well as the activation of a tyrosine phosphorylation signal transduction pathway.<br /> (Copyright 1999 Academic Press.)
- Subjects :
- Animals
Antibodies, Viral immunology
Cells, Cultured
Cytoplasm virology
Kidney virology
Pseudorabies genetics
Swine
Viral Envelope Proteins immunology
Virus Replication genetics
Gene Expression Regulation, Viral
Herpesvirus 1, Suid physiology
Pseudorabies virology
Viral Envelope Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0042-6822
- Volume :
- 259
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 10364498
- Full Text :
- https://doi.org/10.1006/viro.1999.9749