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Using hippocampal slices to study how aging alters ion regulation in brain tissue.
- Source :
-
Methods (San Diego, Calif.) [Methods] 1999 Jun; Vol. 18 (2), pp. 150-9. - Publication Year :
- 1999
-
Abstract
- Aging alters ion regulation in brain tissue. This article describes methods useful for studying such age-related changes in the rat hippocampal slice preparation. Topics considered include (a) selection of appropriate age groups of rats for aging studies, (b) a description of methods for preparing and maintaining hippocampal slices, (c) measurement of intracellular pH with the H+-sensitive dye carboxy-SNARF-1, and (d) measurement of extracellular pH and K+ with cation-selective microelectrodes.<br /> (Copyright 1999 Academic Press.)
- Subjects :
- Animals
Benzopyrans
Calibration
Dissection methods
Electrophysiology instrumentation
Electrophysiology methods
Fluorescent Dyes
Hippocampus growth & development
Homeostasis
Hydrogen-Ion Concentration
In Vitro Techniques
Indicators and Reagents
Male
Microelectrodes
Naphthols
Pyramidal Cells physiology
Rats
Rats, Inbred F344
Rhodamines
Spectrophotometry instrumentation
Spectrophotometry methods
Aging physiology
Hippocampus physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1046-2023
- Volume :
- 18
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Methods (San Diego, Calif.)
- Publication Type :
- Academic Journal
- Accession number :
- 10356345
- Full Text :
- https://doi.org/10.1006/meth.1999.0768