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The quiescent-cell expression system for protein synthesis in Escherichia coli.
- Source :
-
Applied and environmental microbiology [Appl Environ Microbiol] 1999 Jun; Vol. 65 (6), pp. 2710-5. - Publication Year :
- 1999
-
Abstract
- The quiescent-cell expression system is a radical alternative to conventional fermentation for protein overproduction in Escherichia coli. It is dependent on the controlled overexpression of a small RNA called Rcd in hns mutant strains to generate nongrowing, quiescent cells which are not nutrient limited. Quiescent cells no longer produce biomass and have their metabolic resources channelled toward the expression of plasmid-based genes. The biosynthetic capacity of the system is demonstrated by its ability to express chloramphenicol acetyltransferase to more than 40% of total cell protein. Quiescent cells may provide an ideal environment for the expression of toxic as well as benign proteins.
- Subjects :
- Chloramphenicol O-Acetyltransferase biosynthesis
DNA-Binding Proteins genetics
Escherichia coli genetics
Escherichia coli growth & development
Lac Operon
Methionine metabolism
Plasmids
RNA, Bacterial genetics
Sulfur Radioisotopes metabolism
beta-Galactosidase biosynthesis
Bacterial Proteins biosynthesis
DNA-Binding Proteins metabolism
Escherichia coli metabolism
Gene Expression Regulation, Bacterial
RNA, Bacterial metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0099-2240
- Volume :
- 65
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Applied and environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 10347065
- Full Text :
- https://doi.org/10.1128/AEM.65.6.2710-2715.1999