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Biosynthesis of glycosphingolipids in cultured mouse neuroblastoma cells. Precursor-product relationships among sialoglycosphingolipids.
- Source :
-
The Journal of biological chemistry [J Biol Chem] 1976 Dec 10; Vol. 251 (23), pp. 7626-31. - Publication Year :
- 1976
-
Abstract
- The reaction sequence for the biosynthesis of gangliosides by mouse neuroblastoma cells has been investigated by studying the pattern of incorporation of labeled precursors into sialoglycosphingolipids. Cultured NB41A cells incorporated N-[3H]acetylmannosamine into the sialic acid moiety of GM3 in less than 10 min. Labeled GM2 was not detected in cells incubated for less than 30 min, while measurable radioactivity did not appear in GM1 until after 60 to 90 min. Analogous experiments were carried out using [14C]galactose. No significant amount of labeled hexose was incorporated into asialo-GM2 during 60 min of culture. These studies are in accord with results of previous studies on glycosyltransferases of NB41A cells (Kemp, S. F., and Stoolmiller, A. C. (1976), J. Neurochem. 26, 723-732), and further support the concept that the pathway of synthesis of gangliosides proceeds via GM3 leads to GM2 leads to GM1.
- Subjects :
- Animals
Cell Line
Culture Media
G(M1) Ganglioside biosynthesis
G(M2) Ganglioside biosynthesis
G(M3) Ganglioside biosynthesis
Galactose metabolism
Glucose metabolism
Glycosphingolipids metabolism
Hexosamines metabolism
Kinetics
Mice
Sialic Acids metabolism
Glycosphingolipids biosynthesis
Neuroblastoma metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9258
- Volume :
- 251
- Issue :
- 23
- Database :
- MEDLINE
- Journal :
- The Journal of biological chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 1033935