Back to Search
Start Over
Relaxin stimulates glycodelin mRNA and protein concentrations in human endometrial glandular epithelial cells.
- Source :
-
Molecular human reproduction [Mol Hum Reprod] 1999 Apr; Vol. 5 (4), pp. 372-5. - Publication Year :
- 1999
-
Abstract
- Human endometrium is the major organ that produces glycodelin A (GdA). The production of endometrial GdA causes a fluctuation of the peripheral glycodelin concentrations in women during the menstrual cycle and pregnancy. It has recently been reported that the rise of plasma concentrations of glycodelin is correlated with relaxin during the late luteal phase and early pregnancy. In addition, administration of relaxin increases glycodelin plasma concentrations, suggesting that relaxin induces GdA production in endometrium. To investigate whether relaxin regulates the GdA synthesis, human endometrial glandular epithelial cells were isolated and cultured with or without relaxin for up to 4 days. Western blot showed that GdA synthesized and secreted from epithelial glands had a major molecular weight of 28 kDa, i.e. the same as the GdA isolated from amniotic fluid. Cells incubated with relaxin consistently increased in GdA production rate (2-6-fold). The GdA mRNA concentrations increased 2-11-fold in cells incubated with relaxin for 2-4 days, as determined by solution hybridization/ribonuclease protection assay. The increase of the mRNA concentration indicates that relaxin activates GdA transcription.
- Subjects :
- Adult
Blotting, Western
Cells, Cultured
Cyclic AMP metabolism
Endometrium cytology
Endometrium drug effects
Epithelial Cells drug effects
Epithelial Cells metabolism
Female
Glycodelin
Glycoproteins drug effects
Glycoproteins genetics
Humans
Imidazoles pharmacology
Phosphodiesterase Inhibitors pharmacology
Pregnancy Proteins drug effects
Pregnancy Proteins genetics
RNA, Messenger drug effects
Relaxin pharmacology
Ribonucleases metabolism
Endometrium metabolism
Glycoproteins metabolism
Pregnancy Proteins metabolism
RNA, Messenger metabolism
Relaxin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1360-9947
- Volume :
- 5
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Molecular human reproduction
- Publication Type :
- Academic Journal
- Accession number :
- 10321810
- Full Text :
- https://doi.org/10.1093/molehr/5.4.372