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Arachidonoyl-CoA:lysophosphatidylcholine acyltransferase activity in ras-transformed NIH 3T3 fibroblasts depends on the membrane composition.
- Source :
-
Biochemistry and molecular biology international [Biochem Mol Biol Int] 1999 Apr; Vol. 47 (4), pp. 555-61. - Publication Year :
- 1999
-
Abstract
- Investigations were performed on the influence of membrane lipids on arachidonoyl-CoA:lysophosphatidylcholine acyltransferase in microsomal membranes from control and ras-transformed NIH 3T3 fibroblasts. Of all the tested phospholipids only sphingomyelin induced activation of acyltransferase in membranes from ras-transformed cells. No specific phospholipid effect on the acyltransferase was observed in microsomal membranes from control fibroblasts. Diacylglycerol was found to inhibit acyltransferase in both cell lines, whereas ceramide accumulation induced inhibition only in membranes from the transformed cells. The effects of diacylglycerol, ceramide, sphingomyelin and sphingomyelinase are discussed with respect to their putative roles in the signal transduction pathways in oncogene-expressing cells.
- Subjects :
- 3T3 Cells
Animals
Cell Line, Transformed
Humans
Intracellular Membranes drug effects
Membrane Lipids metabolism
Mice
Microsomes drug effects
Phospholipids metabolism
Phospholipids pharmacology
ras Proteins genetics
Acyltransferases metabolism
Intracellular Membranes metabolism
Microsomes metabolism
ras Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1039-9712
- Volume :
- 47
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biochemistry and molecular biology international
- Publication Type :
- Academic Journal
- Accession number :
- 10319406
- Full Text :
- https://doi.org/10.1080/15216549900201593