Back to Search
Start Over
Structure and function prediction of the Brucella abortus P39 protein by comparative modeling with marginal sequence similarities.
- Source :
-
Protein engineering [Protein Eng] 1999 Mar; Vol. 12 (3), pp. 217-23. - Publication Year :
- 1999
-
Abstract
- A methodology is proposed to solve a difficult modeling problem related to the recently sequenced P39 protein. This sequence shares no similarity with any known 3D structure, but a fold is proposed by several threading tools. The difficulty in aligning the target sequence on one of the proposed template structures is overcome by combining the results of several available prediction methods and by refining a rational consensus between them. In silico validation of the obtained model and a preliminary cross-check with experimental features allow us to state that this borderline prediction is at least reasonable. This model raises relevant hypotheses on the main structural features of the protein and allows the design of site-directed mutations. Knowing the genetic context of the P39 reading frame, we are now able to suggest a function for the P39 protein: it would act as a periplasmic substrate-binding protein.
- Subjects :
- Amino Acid Sequence
Carrier Proteins chemistry
Membrane Proteins chemistry
Models, Molecular
Molecular Sequence Data
Open Reading Frames
Protein Structure, Secondary
Sequence Homology, Amino Acid
Structure-Activity Relationship
Bacterial Proteins
Brucella abortus metabolism
Carrier Proteins metabolism
Membrane Proteins metabolism
Periplasmic Binding Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0269-2139
- Volume :
- 12
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Protein engineering
- Publication Type :
- Academic Journal
- Accession number :
- 10235622
- Full Text :
- https://doi.org/10.1093/protein/12.3.217