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Expression of interferon-beta is associated with growth arrest of murine and human epidermal cells.
- Source :
-
The Journal of investigative dermatology [J Invest Dermatol] 1999 May; Vol. 112 (5), pp. 802-9. - Publication Year :
- 1999
-
Abstract
- The cytokine interferon-beta is a regulator of cell replication and function, including invasion and induction of angiogenesis. The goal of this study was to determine whether the expression of interferon-beta by cells in the epidermis correlated with terminal differentiation. In situ hybridization analysis and immunohistochemical staining of formalin-fixed, paraffin-embedded specimens of normal human and murine epidermis and human and murine skin tumors of epithelial origin revealed that only differentiated, nondividing cells of the epidermis expressed interferon-beta protein. Keratinocyte cultures established from the epidermis of 3 d old mice were maintained under conditions permitting continuous cell division or induction of differentiation. Continuously dividing cells did not produce interferon-beta whereas nondividing differentiated cells expressing keratin 1 did. Growth-arrested, undifferentiated keratinocytes also expressed interferon-beta protein. Neutralizing interferon-beta in the culture medium inhibited differentiation, but the addition of exogenous interferon-beta did not stimulate differentiation. These data indicate that interferon-beta is produced by growth-arrested, terminally differentiated keratinocytes.
- Subjects :
- Animals
Antibodies pharmacology
Calcium physiology
Carcinoma, Squamous Cell metabolism
Carcinoma, Squamous Cell pathology
Cell Differentiation
Cell Division physiology
Cells, Cultured
Fluorescent Antibody Technique
Humans
Immunohistochemistry
Interferon-beta immunology
Interferon-beta pharmacology
Interferon-beta physiology
Keratin-14
Keratinocytes cytology
Keratinocytes metabolism
Keratins biosynthesis
Membrane Proteins biosynthesis
Mice
Mice, Inbred BALB C
Mice, Transgenic
Skin Neoplasms metabolism
Skin Neoplasms pathology
Time Factors
Epidermal Cells
Epidermis metabolism
Interferon-beta biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0022-202X
- Volume :
- 112
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The Journal of investigative dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 10233775
- Full Text :
- https://doi.org/10.1046/j.1523-1747.1999.00566.x