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Evaluation of new monoclonal anti-MyoD1 and anti-myogenin antibodies for the diagnosis of rhabdomyosarcoma.
- Source :
-
Pathology international [Pathol Int] 1999 Jan; Vol. 49 (1), pp. 62-8. - Publication Year :
- 1999
-
Abstract
- New monoclonal anti-MyoD1 and anti-myogenin antibodies were evaluated immunohistochemically to determine whether they are useful in discriminating rhabdomyosarcoma (RMS) from other soft tissue tumors in routinely processed sections. Neither MyoD1 nor myogenin was expressed in normal, mature striated muscle. In RMS, nuclear expression of MyoD1 and myogenin was found in 82 and 80% of non-overlapping cases, respectively. MyoD1 was generally expressed in small, primitive tumor cells, and larger cells exhibiting morphological evidence of skeletal muscle differentiation failed to express positive nuclear immunostaining. Positive nuclear staining for myogenin was stronger than that for MyoD1 in cases with abundant differentiated tumor cells, but was less prominent in cases in which small, primitive tumor cells predominated. No leiomyosarcomas, Ewing's sarcomas/peripheral primitive neuroectodermal tumors or other soft tissue tumors exhibited nuclear expression of MyoD1 or myogenin. In conclusion, both anti-MyoD1 and anti-myogenin antibodies are useful for diagnosing RMS and for discriminating RMS from other soft tissue tumors.
- Subjects :
- Actins analysis
Diagnosis, Differential
Humans
Immunohistochemistry
Muscle, Skeletal chemistry
Muscle, Smooth chemistry
MyoD Protein immunology
Myogenin immunology
Rhabdomyosarcoma diagnosis
Sarcomeres chemistry
Soft Tissue Neoplasms diagnosis
Soft Tissue Neoplasms metabolism
Antibodies, Monoclonal analysis
MyoD Protein analysis
Myogenin analysis
Rhabdomyosarcoma metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1320-5463
- Volume :
- 49
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Pathology international
- Publication Type :
- Academic Journal
- Accession number :
- 10227726
- Full Text :
- https://doi.org/10.1046/j.1440-1827.1999.00825.x