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Evidence for the involvement of the Glc7-Reg1 phosphatase and the Snf1-Snf4 kinase in the regulation of INO1 transcription in Saccharomyces cerevisiae.
- Source :
-
Genetics [Genetics] 1999 May; Vol. 152 (1), pp. 73-87. - Publication Year :
- 1999
-
Abstract
- Binding of the TATA-binding protein (TBP) to the promoter is a pivotal step in RNA polymerase II transcription. To identify factors that regulate TBP, we selected for suppressors of a TBP mutant that exhibits promoter-specific defects in activated transcription in vivo and severely reduced affinity for TATA boxes in vitro. Dominant mutations in SNF4 and recessive mutations in REG1, OPI1, and RTF2 were isolated that specifically suppress the inositol auxotrophy of the TBP mutant strains. OPI1 encodes a repressor of INO1 transcription. REG1 and SNF4 encode regulators of the Glc7 phosphatase and Snf1 kinase, respectively, and have well-studied roles in glucose repression. In two-hybrid assays, one SNF4 mutation enhances the interaction between Snf4 and Snf1. Suppression of the TBP mutant by our reg1 and SNF4 mutations appears unrelated to glucose repression, since these mutations do not alleviate repression of SUC2, and glucose levels have little effect on INO1 transcription. Moreover, mutations in TUP1, SSN6, and GLC7, but not HXK2 and MIG1, can cause suppression. Our data suggest that association of TBP with the TATA box may be regulated, directly or indirectly, by a substrate of Snf1. Analysis of INO1 transcription in various mutant strains suggests that this substrate is distinct from Opi1.
- Subjects :
- AMP-Activated Protein Kinases
Blotting, Northern
Cell Cycle Proteins genetics
Chromatin metabolism
Genomic Library
Genotype
Glycerol pharmacology
Glycoside Hydrolases metabolism
Lactic Acid pharmacology
Mutagenesis
Plasmids
Protein Phosphatase 1
RNA Polymerase II metabolism
Raffinose pharmacology
Suppression, Genetic genetics
TATA-Box Binding Protein analogs & derivatives
Time Factors
Transcription, Genetic
Tubulin metabolism
beta-Fructofuranosidase
beta-Galactosidase metabolism
Carrier Proteins
Fungal Proteins physiology
Gene Expression Regulation, Fungal
Myo-Inositol-1-Phosphate Synthase genetics
Phosphoprotein Phosphatases physiology
Protein Kinases physiology
Protein Serine-Threonine Kinases physiology
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins
Transcription Factors physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0016-6731
- Volume :
- 152
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Genetics
- Publication Type :
- Academic Journal
- Accession number :
- 10224244
- Full Text :
- https://doi.org/10.1093/genetics/152.1.73