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Acid-induced acid tolerance and acidogenicity of non-mutans streptococci.
- Source :
-
Oral microbiology and immunology [Oral Microbiol Immunol] 1999 Feb; Vol. 14 (1), pp. 43-8. - Publication Year :
- 1999
-
Abstract
- Acid tolerance and acidogenicity of non-mutans streptococci and their capacity of acid adaptation were studied. The cells of non-mutans streptococci (Streptococcus sanguis [Streptococcus sanguinis], Streptococcus gordonii, Streptococcus oralis and Streptococcus mitis) grown at pH 7.0 showed 0.0088% to 71% viability after acidification at pH 4.0 for 60 min, whereas the cells of mutans streptococci (Streptococcus mutans) were not killed by the acidification. Washed cells of non-mutans streptococci lowered pH to 4.04-4.33 in the presence of glucose, while mutans streptococci cells lowered pH to 3.70. When the growth pH was shifted to 5.5 for 30-90 min, the viability of non-mutans streptococci after the acidification at pH 4.0 for 60 min increased (0.25% to 91%) and the minimum pH values of the cells in the presence of glucose decreased (3.90 4.19). Along with the increase in acid tolerance and acidogenicity, non-mutans streptococci increased activities of H(+)-ATPase and arginine deiminase and amounts of stress proteins cross-reacting with 60 kDa and 70 kDa heat shock proteins (Hsp60 and Hsp70). These results indicate that non-mutans streptococci were capable of increasing their acid tolerance and acidogenicity in response to environmental acidification. Furthermore, it is suggested that the acid adaptation observed in non-mutans streptococci cells could involve the induction of H(+)-ATPase, arginine deiminase and stress protein syntheses. The strains of non-mutans streptococci, which are pioneer bacteria for dental plaque formation and predominant in plaque microbial flora, may play a significant role in shifting the dental plaque environment toward acidic and consequently promoting the colonization of more acid-tolerant and acidogenic bacteria such as mutans streptococci and lactobacilli.
- Subjects :
- Adaptation, Physiological
Bacterial Proteins metabolism
Culture Media
Glycolysis
Heat-Shock Proteins metabolism
Hydrogen-Ion Concentration
Hydrolases metabolism
Proton-Translocating ATPases metabolism
Streptococcus chemistry
Streptococcus oralis chemistry
Streptococcus oralis enzymology
Streptococcus oralis physiology
Streptococcus sanguis enzymology
Streptococcus sanguis physiology
Acids metabolism
Streptococcus enzymology
Streptococcus physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0902-0055
- Volume :
- 14
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Oral microbiology and immunology
- Publication Type :
- Academic Journal
- Accession number :
- 10204479
- Full Text :
- https://doi.org/10.1034/j.1399-302x.1999.140105.x