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Measurement of bile salt hydrolase activity from Lactobacillus acidophilus based on disappearance of conjugated bile salts.
- Source :
-
Journal of dairy science [J Dairy Sci] 1999 Mar; Vol. 82 (3), pp. 466-71. - Publication Year :
- 1999
-
Abstract
- Bile salt hydrolase activity of Lactobacillus acidophilus was measured based on the disappearance of sodium glycocholate and sodium taurocholate from the reaction mixture using HPLC. The amount of sodium glycocholate and sodium taurocholate that disappeared was proportional to the amount of sodium cholate that appeared in the mixture as detected by HPLC. Sodium glycocholate did not precipitate at the enzyme reaction conditions (37 degrees C and pH 5.4) for determining bile salt hydrolase activity. The bile salt hydrolase assay was insensitive to low oxidation-reduction potential when measuring bile salt hydrolase from L. acidophilus, an intestinal microorganism. However, EDTA and freezing temperatures were necessary to maintain stability of the partially purified enzyme during storage.
Details
- Language :
- English
- ISSN :
- 0022-0302
- Volume :
- 82
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of dairy science
- Publication Type :
- Academic Journal
- Accession number :
- 10194663
- Full Text :
- https://doi.org/10.3168/jds.S0022-0302(99)75255-0