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Target joining of duplicated insertion sequence IS21 is assisted by IstB protein in vitro.
- Source :
-
Journal of bacteriology [J Bacteriol] 1999 Apr; Vol. 181 (7), pp. 2286-9. - Publication Year :
- 1999
-
Abstract
- Tandemly repeated insertion sequence IS21, located on a suicide plasmid, promoted replicon fusion with bacteriophage lambda in vitro in the presence of ATP. This reaction was catalyzed in a cell extract containing the 45-kDa IstA protein (cointegrase) and the 30-kDa IstB helper protein of IS21 after both proteins had been overproduced in Escherichia coli. Without IstB, replicon fusion was inefficient and did not produce the 4-bp target duplications typical of IS21.
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 181
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 10094711
- Full Text :
- https://doi.org/10.1128/JB.181.7.2286-2289.1999