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Enumeration of antigen-presenting cells in mice infected with Sendai virus.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 1999 Mar 15; Vol. 162 (6), pp. 3350-5. - Publication Year :
- 1999
-
Abstract
- Substantial progress has been made in understanding Ag presentation to T cells; however, relatively little is known about the location and frequency of cells presenting viral Ags during a viral infection. Here, we took advantage of a highly sensitive system using lacZ-inducible T cell hybridomas to enumerate APCs during the course of respiratory Sendai virus infection in mice. Using lacZ-inducible T cell hybridomas specific for the immunodominant hemagglutinin-neuraminidase HN421-436/I-Ab and nucleoprotein NP324-332/Kb epitopes, we detected APCs in draining mediastinal lymph nodes (MLNs), in cervical lymph nodes, and also in the spleen. HN421-436/I-Ab- and NP324-332/Kb-presenting cells were readily detectable between days 3 and 9 postinfection, with more APCs present in the MLN than in the cervical lymph nodes. Interestingly, no infectious virus was detected in lymphoid tissue beyond day 6, suggesting that a depot of noninfectious viral Ag survives, in some form, for 2-3 days after viral clearance. Fractionation of the MLN demonstrated that APC frequency was enriched in dendritic cells and macrophages but depleted in the B cell population, suggesting that B cells do not form a large population of APCs during the primary response to this virus.
- Subjects :
- Amino Acid Sequence
Animals
Antigen-Presenting Cells immunology
Antigen-Presenting Cells virology
Antigens, Viral metabolism
Epitopes, T-Lymphocyte metabolism
Female
Hybridomas cytology
Hybridomas virology
Immunophenotyping
Lac Operon immunology
Lymphocyte Activation genetics
Lymphoid Tissue cytology
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Respirovirus Infections metabolism
T-Lymphocytes cytology
T-Lymphocytes immunology
T-Lymphocytes virology
Antigen-Presenting Cells cytology
Cell Count methods
Respirovirus immunology
Respirovirus Infections immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 162
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 10092789