Back to Search
Start Over
Molecular cloning of the human ATP-binding cassette transporter 1 (hABC1): evidence for sterol-dependent regulation in macrophages.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1999 Apr 02; Vol. 257 (1), pp. 29-33. - Publication Year :
- 1999
-
Abstract
- We have cloned the full-length cDNA for the human ATP binding cassette transporter 1 (hABC1). The 6603-bp open reading frame encodes a polypeptide of 2201 amino acids resulting in a deduced molecular weight of 220 kDa. The hABC1 cDNA is highly homologous (62%) to the human rim ABC transporter (ABCR). hABC1 is expressed in a variety of human tissues with highest expression levels found in placenta, liver, lung, adrenal glands, and fetal tissues. We demonstrate that the hABC1 expression is induced during differentiation of human monocytes into macrophages in vitro. In macrophages, both the hABC1 mRNA and protein expression are upregulated in the presence of acetylated low-density lipoprotein (AcLDL). The AcLDL-induced increase in hABC1 expression is reversed by cholesterol depletion mediated by the addition of high-density lipoprotein (HDL3). Our data, demonstrating sterol-dependent regulation of hABC1 in human monocytes/macrophages, suggest a novel role for this transporter molecule in membrane lipid transport.<br /> (Copyright 1999 Academic Press.)
- Subjects :
- ATP Binding Cassette Transporter 1
ATP-Binding Cassette Transporters chemistry
ATP-Binding Cassette Transporters metabolism
Amino Acid Sequence
Blotting, Northern
Blotting, Western
Cell Differentiation drug effects
Cells, Cultured
Cloning, Molecular
DNA, Complementary genetics
Glycoproteins chemistry
Glycoproteins metabolism
Humans
Lipoproteins, HDL metabolism
Lipoproteins, HDL pharmacology
Lipoproteins, LDL antagonists & inhibitors
Lipoproteins, LDL metabolism
Lipoproteins, LDL pharmacology
Macrophage Colony-Stimulating Factor pharmacology
Macrophages drug effects
Molecular Sequence Data
Molecular Weight
Monocytes drug effects
Monocytes metabolism
Open Reading Frames genetics
RNA, Messenger metabolism
Sequence Homology, Amino Acid
ATP-Binding Cassette Transporters genetics
Gene Expression Regulation drug effects
Glycoproteins genetics
Macrophages metabolism
Sterols metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 257
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 10092505
- Full Text :
- https://doi.org/10.1006/bbrc.1999.0406