Back to Search
Start Over
Two new glucose 6-phosphate dehydrogenase variants associated with congenital nonspherocytic hemolytic anemia found in Japan: GD(-) Tokushima and GD(-) Tokyo.
- Source :
-
American journal of hematology [Am J Hematol] 1976; Vol. 1 (4), pp. 433-42. - Publication Year :
- 1976
-
Abstract
- Two new variants of glucose 6-phosphate dehydrogenase (G6PD) deficiency associated with chronic nonspherocytic hemolytic anemia were discovered in Japan. Gd(-) Tokushima was found in a 17-years-old male whose erythrocytes contained 4.4% of normal enzyme activity. Partially purified enzyme revealed a main band of normal electrophoretic mobility with additional two minor bands of different mobility; normal Km G6P, and Km NADP five-to sixfold higher than normal; normal utilization of 2-deoxy-G6P, galactose-6P, and deamino-NADP; marked thermal instability; a normal pH curve; and normal Ki NADPH. The hemolytic anemia was moderate to severe. Gd(-) Tokyo was characterized from a 15-year-old male who had chronic nonspherocytic hemolytic anemia of mild degree. The erythrocytes contained 3% of normal enzyme activity, and partially purified enzyme revealed slow electrophoretic mobility (90% of normal for both a tris-hydrochloride buffer system and a tris-EDTA-borate buffer system, and 70% of normal for a phosphate buffer system); normal Km G6P and Km NADP; normal utilization of 2-deoxy-G6P, galactose-6P, and deamino-NADP; greatly increased thermal instability; a normal pH curve; and normal Ki NADPH. These two variants are clearly different from hitherto described G6PD variants, including the Japanese variants Gd(-) Heian and Gd(-) Kyoto. The mothers of both Gd(-) Tokushima and Gd(-) Tokoyo were found to be heterozygote by an ascorbate-cyanide test.
- Subjects :
- Adolescent
Female
Glucosephosphate Dehydrogenase Deficiency blood
Glucosephosphate Dehydrogenase Deficiency genetics
Humans
Japan
Male
Tokyo
Anemia, Hemolytic, Congenital Nonspherocytic complications
Genetic Variation
Glucosephosphate Dehydrogenase blood
Glucosephosphate Dehydrogenase Deficiency complications
Subjects
Details
- Language :
- English
- ISSN :
- 0361-8609
- Volume :
- 1
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- American journal of hematology
- Publication Type :
- Academic Journal
- Accession number :
- 1008056
- Full Text :
- https://doi.org/10.1002/ajh.2830010408