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Requirement of expression of P-glycoprotein on human natural killer leukemia cells for cell-mediated cytotoxicity.
- Source :
-
Biochemical pharmacology [Biochem Pharmacol] 1998 May 01; Vol. 55 (9), pp. 1385-90. - Publication Year :
- 1998
-
Abstract
- The requirement of P-glycoprotein, a product of the multidrug resistance (MDR)1 gene, for natural killer (NK) cell-mediated cytotoxicity was examined by using a human NK-like cell line, YTN, which is cytotoxic toward JY cells. YTN cells express P-glycoprotein, a judged by flow cytometry and polymerase chain reaction of reverse-transcribed mRNA. YTN cell-mediated cytotoxicity was inhibited by MDR-reversing reagents as well as the F(ab')2 fragment of a monoclonal antibody against P-glycoprotein. Furthermore, antisense oligonucleotides for MDR1 mRNA inhibited expression of P-glycoprotein as well as YTN cell-mediated cytotoxicity. Thus, this study provides firm evidence that P-glycoprotein plays an essential role in cell-mediated cytotoxicity.
- Subjects :
- ATP Binding Cassette Transporter, Subfamily B, Member 1 metabolism
Cell Line
Cell Survival drug effects
Cytotoxicity, Immunologic drug effects
Dihydropyridines pharmacology
Humans
Nicardipine pharmacology
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Pyrazoles pharmacology
RNA, Messenger genetics
Thymoma
Thymus Neoplasms
Transcription, Genetic
Tumor Cells, Cultured
ATP Binding Cassette Transporter, Subfamily B, Member 1 genetics
Cytotoxicity, Immunologic genetics
Drug Resistance, Multiple genetics
Killer Cells, Natural immunology
Oligonucleotides, Antisense pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-2952
- Volume :
- 55
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Biochemical pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 10076529
- Full Text :
- https://doi.org/10.1016/s0006-2952(97)00652-7