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Heterogeneity and susceptibility to apoptosis of human renal carcinoma cells in vitro.
- Source :
-
European journal of histochemistry : EJH [Eur J Histochem] 1998; Vol. 42 (4), pp. 327-32. - Publication Year :
- 1998
-
Abstract
- Tumors are heterogeneous in terms of morphology and susceptibility to drugs or radiation. Among primary and metastatic cells of a human renal carcinoma, a population (type II) of larger cells with prominent nucleoli, eosinophilic globules of intermediate filaments in paranuclear bundles, margination of subcellular organelles and peripheral pools of glycogen was evident. Paranuclear structures were recognized by monoclonal antibodies specific for cytokeratin 8, 18 and 19, but not by vimentin specific antibodies. We propagated a cell line in vitro (referred to as BKR cells), and observed culture in vitro, the almost complete disappearance of the type II cells. Pharmacological agents that influence cell differentiation, such as retinoic acid, rescued the expression of type II cells in vitro. Long-term treatments with insulin or alpha-interferon, but not with the epithelial growth factor (EGF), similarly differentiated BKR cells and abated their susceptibility to spontaneous and actinomycin-D induced apoptosis. These data support the contention that differentiation of tumor cells is actively maintained in vivo and further strengthen the caveat on tumor lines stabilized in vitro, that poorly represent the morphologic and antigenic heterogeneity of neoplasms in vivo.
- Subjects :
- Blotting, Western
Bone Neoplasms metabolism
Carcinoma, Renal Cell metabolism
Carcinoma, Renal Cell secondary
Carcinoma, Renal Cell ultrastructure
Cell Size drug effects
Fas Ligand Protein
Humans
Immunohistochemistry
Insulin pharmacology
Kidney Neoplasms metabolism
Kidney Neoplasms ultrastructure
Membrane Glycoproteins metabolism
Microscopy, Electron
Phenotype
Tretinoin pharmacology
Tumor Cells, Cultured
Apoptosis
Bone Neoplasms secondary
Carcinoma, Renal Cell pathology
Kidney Neoplasms pathology
Sacrum
Subjects
Details
- Language :
- English
- ISSN :
- 1121-760X
- Volume :
- 42
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- European journal of histochemistry : EJH
- Publication Type :
- Academic Journal
- Accession number :
- 10068906