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Substance P receptor (neurokinin-1)-expressing neurons in lamina I of the spinal cord encode for the intensity of noxious stimulation: a c-Fos study in rat.
- Source :
-
Neuroscience [Neuroscience] 1999 Mar; Vol. 89 (1), pp. 17-28. - Publication Year :
- 1999
-
Abstract
- The substance P receptor neurokinin-1 is expressed by a subset of neurons in the rat spinal cord. We have combined immunostaining for Fos, a marker of noxious peripheral stimulation, and neurokinin-1 to examine whether nociceptive signals from particular peripheral tissues (skin, muscle or knee joint) or activity generated by nerve injury or formalin-induced inflammation are preferentially modulated by substance P. Our results indicate that superficial and deep spinal neurokinin-1-positive neurons process nociceptive information in markedly different ways. In lamina I, the number of double-labelled neurons was positively correlated with the intensity of the stimulus (defined by the total Fos count) and was not directly related to any particular peripheral target. However, in the deeper layers of the spinal cord (V-X), there was no such correlation, and stimulation of joint nociceptors and formalin-induced inflammation produced the greatest proportion of Fos/neurokinin-1 co-localization, suggesting a particular role for substance P in the mediation of joint pain and inflammatory hyperalgesia. Thus, lamina I neurokinin-1 receptor-bearing neurons appear to be involved in intensity discriminative aspects of pain, whereas the deep neurokinin-1 cells are involved in spatial localization or the detection of particular nociceptive submodalities.
- Subjects :
- Animals
Antibodies
Gene Expression physiology
Hindlimb
Hot Temperature
Male
Muscle, Skeletal innervation
Mustard Plant
Nerve Crush
Neurons drug effects
Neurons physiology
Nociceptors drug effects
Nociceptors physiology
Pain physiopathology
Plant Extracts
Plant Oils
Rats
Rats, Sprague-Dawley
Receptors, Neurokinin-1 analysis
Receptors, Neurokinin-1 immunology
Sciatic Nerve chemistry
Sciatic Nerve cytology
Sciatic Nerve injuries
Spinal Cord chemistry
Spinal Cord physiology
Stimulation, Chemical
Neurogenic Inflammation physiopathology
Neurons chemistry
Proto-Oncogene Proteins c-fos genetics
Receptors, Neurokinin-1 genetics
Spinal Cord cytology
Subjects
Details
- Language :
- English
- ISSN :
- 0306-4522
- Volume :
- 89
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 10051214
- Full Text :
- https://doi.org/10.1016/s0306-4522(98)00276-0