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Rapid arrest of axon elongation by brefeldin A: a role for the small GTP-binding protein ARF in neuronal growth cones.
- Source :
-
Journal of neurobiology [J Neurobiol] 1999 Jan; Vol. 38 (1), pp. 105-15. - Publication Year :
- 1999
-
Abstract
- Members of the ADP-ribosylation factor (ARF) family of small guanosine triphosphate-binding proteins play an essential role in membrane trafficking which subserves constitutive protein transport along exocytic and endocytic pathways within eukaryotic cell bodies. In growing neurons, membrane trafficking within motile growth cones distant from the cell body underlies the rapid plasmalemmal expansion which subserves axon elongation. We report here that ARF is a constituent of axonal growth cones, and that application of brefeldin A to neurons in culture produces a rapid arrest of axon extension that can be ascribed to inhibition of ARF function in growth cones. Our findings demonstrate a role for ARF in growth cones that is coupled tightly to the rapid growth of neuronal processes characteristic of developmental and regenerative axon elongation, and indicate that ARF participates not only in constitutive membrane traffic within the cell body, but also in membrane dynamics within growing axon endings.
- Subjects :
- ADP-Ribosylation Factors
Animals
Animals, Newborn
Axons
Carrier Proteins antagonists & inhibitors
Carrier Proteins physiology
GTP-Binding Proteins physiology
Growth Cones physiology
Neurites physiology
Rats
Brefeldin A pharmacology
GTP-Binding Proteins antagonists & inhibitors
Growth Cones drug effects
Neurites drug effects
Protein Synthesis Inhibitors pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3034
- Volume :
- 38
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of neurobiology
- Publication Type :
- Academic Journal
- Accession number :
- 10027566
- Full Text :
- https://doi.org/10.1002/(sici)1097-4695(199901)38:1<105::aid-neu8>3.0.co;2-m