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Spectroscopic investigation on the interaction of ruthenium complexes with tumor specific lectin, jacalin.

Spectroscopic investigation on the interaction of ruthenium complexes with tumor specific lectin, jacalin.

Authors :
Ayaz Ahmed, Khan Behlol
Reshma, Elamvazhuthi
Mariappan, Mariappan
Anbazhagan, Veerappan
Source :
Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy. Feb2015, Vol. 137, p1292-1297. 6p.
Publication Year :
2015

Abstract

Several ruthenium complexes are regarded as anticancer agents and considered as an alternative to the widely used platinum complexes. Owing to the preferential interaction of jacalin with tumor-associated T-antigen, we report the interaction of jacalin with four ruthenium complex namely, tris(1,10-phenanthroline)ruthenium(II)chloride, bis(1,10-phenanthroline)(N-[1,10]phenanthrolin-5-yl-pyrenylmethanimine)ruthenium(II)chloride, bis(1,10-phenanthroline)(dipyrido[3,2- a :2′,3′- c ]-phenazine)ruthenium(II)chloride, bis(1,10-phenanthroline)(11-(9-acridinyl)dipyrido[3,2-a:2′,3′-c]phenazine)ruthenium(II) chloride. Fluorescence spectroscopic analysis revealed that the ruthenium complexes strongly quenched the intrinsic fluorescence of jacalin through a static quenching procedure, and a non-radiative energy transfer occurred within the molecules. Association constants obtained for the interaction of different ruthenium complexes with jacalin are in the order of 10 5 M −1 , which is in the same range as those obtained for the interaction of lectin with carbohydrate and hydrophobic ligand. Each subunit of the tetrameric jacalin binds one ruthenium complex, and the stoichiometry is found to be unaffected by the presence of the specific sugar, galactose. In addition, agglutination activity of jacalin is largely unaffected by the presence of the ruthenium complexes, indicating that the binding sites for the carbohydrate and the ruthenium complexes are different. These results suggest that the development of lectin–ruthenium complex conjugate would be feasible to target malignant cells in chemo-therapeutics. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
13861425
Volume :
137
Database :
Academic Search Index
Journal :
Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy
Publication Type :
Academic Journal
Accession number :
99830778
Full Text :
https://doi.org/10.1016/j.saa.2014.09.047