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Determination of major metabolites of MAM-2201 and JWH-122 in in vitro and in vivo studies to distinguish their intake.
- Source :
-
Forensic Science International . 2014, Vol. 244, p85-91. 7p. - Publication Year :
- 2014
-
Abstract
- Abuse of fluorinated synthetic cannabinoid analogs to avoid existing legal regulations has increased globally. The fluorinated JWH-122 analog, MAM-2201, was first reported in September 2012 as an ingredient in herbal mixtures in Korea. MAM-2201 is more potent than JWH-122 and a fatal intoxication case has been reported. In this study, we identified major MAM-2201 and JWH-122 metabolites from in vitro metabolism studies using human liver microsomes and compared the results with those of urine specimens from suspected MAM-2201 or JWH-122 users. MAM-2201 and JWH-122 produced common metabolites, N-5-hydroxylated, N-4-hydroxylated and carboxylated JWH-122 metabolites. Trace amounts of an N-4-hydroxylated MAM-2201 metabolite, a characteristic MAM-2201 metabolite, was detected in only a few urine specimens from MAM-2201 users. Both in vitro and in vivo studies demonstrated that N-5-hydroxylated JWH-122 metabolite was the primary metabolite of MAM-2201, whereas N-4-hydroxylated JWH-122 metabolite was predominant in JWH-122 metabolism. Based on these results, relative concentrations of N-5- and N-4-hydroxylated JWH-122 metabolites should be considered to verify MAM-2201 or JWH-122 users. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CANNABINOIDS
*FLUORINATION
*MIXTURES
*METABOLISM
*URINALYSIS
Subjects
Details
- Language :
- English
- ISSN :
- 03790738
- Volume :
- 244
- Database :
- Academic Search Index
- Journal :
- Forensic Science International
- Publication Type :
- Academic Journal
- Accession number :
- 99390000
- Full Text :
- https://doi.org/10.1016/j.forsciint.2014.08.008