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Antiviral and cytotoxic evaluation of coumarins from Prangos ferulacea.
- Source :
-
Pharmaceutical Biology . Dec2014, Vol. 52 Issue 12, p1543-1549. 7p. - Publication Year :
- 2014
-
Abstract
- Context: Prangos ferulacea (L.) Lindl. (Apiaceae) is a perennial plant found in the Middle-East, where it is commonly used as an antispasmodic and anti-inflammatory agent. It is a rich source of coumarins. Objective: To purify several coumarins from P. ferulacea and to screen their cytotoxicity and anti-herpes activity. Materials and methods: Acetone extract of roots of P. ferulacea was subjected to several chromatographic separations to render pure coumarins ( 1-8). Anti-herpes virus effects of 1- 7 were evaluated at concentration 2.5, 5, and 10 µgmL−1, on a confluent monolayer of Vero cells infected with 25 PFU of HSV1. Cytotoxic effects of 1 and 2 were evaluated on an A2780S cell line using the MTT assay. The cells were exposed to a series of concentrations of coumarins (0.01-2.5 mM, 37°C, 72 h). Results: Compounds 1- 8 were identified as osthole, isoimperatorin, oxypeucedanin, psoralen, oxypeucedanin hydrate, gosferol, oxypeucedanin methnolate, and pranferol. This is the first report of occurrence of 4 and 7 in this plant. Compound 1 showed a viability of 9.41% ± 2.4 at 2.5 mM on A2780S cells (IC50 = 0.38 mM). The cell survival of 2 at 2.5 mM was 46.86% ± 5.5 with IC50 equal to 1.1 mM. Discussion and conclusion: Compound 1 shows cytotoxic effects on the A2780S cell line. Compound 2 is a cyclooxygenase-2 inhibitor and the A2780S cell line does not express COX-2 which may interpret the non-toxic effect of the compound on this cell line. None of the tested compounds showed an anti-HSV effect at non-toxic concentrations. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13880209
- Volume :
- 52
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Pharmaceutical Biology
- Publication Type :
- Academic Journal
- Accession number :
- 99255111
- Full Text :
- https://doi.org/10.3109/13880209.2014.907322