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Smartphone-Based Simultaneous pH and Nitrite Colorimetric Determination for Paper Microfluidic Devices.
- Source :
-
Analytical Chemistry . 10/7/2014, Vol. 86 Issue 19, p9554-9162. 9p. - Publication Year :
- 2014
-
Abstract
- In this work, an Android application for measurement of nitrite concentration and pH determination in combination with a low-cost paper-based microfluidic device is presented. The application uses seven sensing areas, containing the corresponding immobilized reagents, to produce selective color changes when a sample solution is placed in the sampling area. Under controlled conditions of light, using the flash of the smartphone as a light source, the image captured with the built-in camera is processed using a customized algorithm for multidetection of the colored sensing areas. The developed image-processing allows reducing the influence of the light source and the positioning of the microfluidic device in the picture. Then, the H (hue) and S (saturation) coordinates of the HSV color space are extracted and related to pH and nitrite concentration, respectively. A complete characterization of the sensing elements has been carried out as well as a full description of the image analysis for detection. The results show good use of a mobile phone as an analytical instrument. For the pH, the resolution obtained is 0.04 units of pH, 0.09 of accuracy, and a mean squared error of 0.167. With regard to nitrite, 0.51% at 4.0 mg L-1 of resolution and 0.52 mg L-1 as the limit of detection was achieved. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MICROFLUIDIC devices
*SMARTPHONES
*ALGORITHMS
*IMAGE analysis
Subjects
Details
- Language :
- English
- ISSN :
- 00032700
- Volume :
- 86
- Issue :
- 19
- Database :
- Academic Search Index
- Journal :
- Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 99022848
- Full Text :
- https://doi.org/10.1021/ac5019205