Quantification of alkali-labile phosphate groups in the plasma of Oreochromis niloticus exposed to intermittent discharges of oestrogens: effect of concentration vs. load.

Vitellogenin protein (Vtg) inOreochromis niloticusplasma has been indirectly quantified through protein-bound phosphate groups also known as alkali-labile phosphates (ALP) using a recently modified method. Such method as described in the literature was originally applied to Crucian carp and resulted in lower detection limits (3.2 μg PO43–per mL). In this study,O. niloticusmales were exposed to intermittent doses of oestrogens for 15 days using different concentrations (converted to loads) of 17α-ethinyloestradiol (EE2) (two different aquarium volumes), oestrone (E1) and 17β-oestradiol (E2) individually and in combination (1:1:1). The induction of physiologic and genotoxic effects in erythrocytes was investigated. For the tested oestrogen (EE2), load proved to be more relevant than concentration in determining the oestrogenicity.O. niloticusmales proved to have lower ALP baseline (4.11 µg PO43−/mL plasma, IQ25 = 3.38; IQ75 = 5.18) than other fish species, including Crucian carp, which makes it suitable for oestrogenicity detection in water. Exposure to E2, EE2 separately and in combination (1:1:1) all induced significant increases in the ALP levels at loads ≥ 0.72 μg/fish. This load was three times lower than the E1 load required to increase ALP (≥ 2.2 μg/fish). All oestrogens with loads ≥ 0.072 μg/fish caused significant increase in micronucleus frequency (≥ 2‰) compared with the control (0.1 ± 0.4‰). The study highlighted the importance of taking into account not only concentration and dose regime but also the mass load and therefore, the volume used in the experimental units, which is rarely addressed in ecotoxicity assays. Considering the good sensitivity ofO. niloticusexposed to relatively low concentrations of oestrogens, the combination of the ALP method with auxiliary biomarkers (particularly micronucleus) can be used as a protocol for oestrogenicity and genotoxicity detection in different contaminated waters as part of water environmental monitoring programmes. [ABSTRACT FROM PUBLISHER]