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Viabilidade do sêmen de tatus-peba (Euphractus sexcinctus) centrifugado e diluído em Tris ou agua de coco em pó.

Authors :
da Cunha Sousa, Patrícia
Araujo dos Santos, Erika Aparecida
Maria Silva, Andréia
de Souza Castelo, Thibério
Xavier Peixoto, Gislayne Cristhiane
Alves Freitas, Carlos Iberê
Rodrigues Silva, Alexandre
Source :
Ciência Rural. set2014, Vol. 44 Issue 9, p1645-1650. 6p.
Publication Year :
2014

Abstract

The effects of the centrifugation associated to the use of two extenders on the viability of six-banded armadillo's (Euphractus sexcinctus) sperm were evaluated during a thermo resistance test (TRT). Semen samples (n=12) derived from 04 stud males collected by electroejaculation were divided in four aliquots; two of that were immediately diluted in Tris or powdered coconut water (ACP-119®); the two others were centrifuged (800g 10min-1) prior to the dilution. Samples were incubated at 34°C per 3h, and the semen parameters were evaluated at each hour. In general, dilution promoted a reduction in semen viscosity in the use of both diluents using centrifugation or not. At 60min, a decrease was verified for all semen parameters (P<0.05), however they were better preserved in the use of Tris when compared to ACP-119® (P<0.05) up to 120min. After that, both diluents equated. In addition, centrifugation procedure presented a deleterious effect on the armadillo s semen quality during all the thermoresistance test. In the present conditions, conclude that Tris extender is more efficient than ACP-119® for the preservation of six-banded armadillo semen viability, and the previous centrifugation is unnecessary. [ABSTRACT FROM AUTHOR]

Details

Language :
Portuguese
ISSN :
01038478
Volume :
44
Issue :
9
Database :
Academic Search Index
Journal :
Ciência Rural
Publication Type :
Academic Journal
Accession number :
99003391
Full Text :
https://doi.org/10.1590/0103-8478cr20131015