Back to Search
Start Over
Upregulation of KCa3.1 K+ channel in mesenteric lymph node CD4+T lymphocytes from a mouse model of dextran sodium sulfate-induced inflammatory bowel disease.
- Source :
-
American Journal of Physiology: Gastrointestinal & Liver Physiology . May2014, Vol. 306 Issue 10, pG873-G885. 13p. - Publication Year :
- 2014
-
Abstract
- The intermediate- conductance Ca2+-activated K+ channel KCa3.1/KCNN4 plays an important role in the modulation of Ca2+ signaling through the control of the membrane potential in T lymphocytes. Here, we study the involvement of KCa3.1 in the enlargement of the mesenteric lymph nodes (MLNs) in a mouse model of inflammatory bowel disease (IBD). The mouse model of IBD was prepared by exposing male C57BL/6J mice to 5% dextran sulfate sodium for 7 days. Inflammation-induced changes in KCa3.1 activity and the expressions of KCa3.1 and its regulators in MLN CD4+ T lymphocytes were monitored by real-time PCR, Western blot, voltage-sensitive dye imaging, patch-clamp, and flow cytometric analyses. Concomitant with an upregulation of KCa3.1a and nucleoside diphosphate kinase B (NDPK-B), a positive KCa3.1 regulator, an increase in KCa3.1 activity was observed in MLN CD4+ T lymphocytes in the IBD model. Pharmacological blockade of KCa3.1 elicited the following results: 1) a significant decrease in IBD disease severity, as assessed by diarrhea, visible fecal blood, inflammation, and crypt damage of the colon and MLN enlargement compared with control mice, and 2) the restoration of the expression levels of KCa3.1a, NDPK-B, and Th1 cytokines in IBD model MLN CD4+ T lymphocytes. These findings suggest that the increase in KCa3.1 activity induced by the upregulation of KCa3.1a and NDPK-B may be involved in the pathogenesis of IBD by mediating the enhancement of the proliferative response in MLN CD4+ T lymphocyte and, therefore, that the pharmacological blockade of KCa3.1 may decrease the risk of IBD. [ABSTRACT FROM AUTHOR]
- Subjects :
- *LYMPHOCYTES
*T cells
*LYMPH nodes
*INFLAMMATORY bowel diseases
*DEXTRAN sulfate
Subjects
Details
- Language :
- English
- ISSN :
- 01931857
- Volume :
- 306
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- American Journal of Physiology: Gastrointestinal & Liver Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 98542065
- Full Text :
- https://doi.org/10.1152/ajpgi.00156.2013