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Fabrication and Characterization of a K+-Selective Nanoelectrode and Simultaneous Imaging of Topography and Local K+ Flux Using Scanning Electrochemical Microscopy.
- Source :
-
Analytical Chemistry . 9/2/2014, Vol. 86 Issue 17, p8547-8552. 6p. - Publication Year :
- 2014
-
Abstract
- A nanopipette containing a solution of bis(benzo-15-crown-5) dissolved in 1,6-dichlorohexane was used as an ion-selective electrode (ISE) to probe K+ for shear force-based constant-distance scanning electrochemical microscopy (SECM). In a previous study, the ISE responded only at low K+ concentrations ([K+] < 1 mM), due to the depletion of the bis(benzo-15-crown-5) at the oil/water interface at high K+ concentrations and the unstable response of the tip at the oil/water interface for shear force and current detection. In the present study, a nanopipette reshaped by heating and with the hydrophobic layer removed was used as the ISE. This modified ISE enabled a rapid response to changes in K+ flux at a physiological concentration of K+ and allowed SECM imaging on a nanometer scale. The fabricated nano-ISE was used as a probe for shear force-based SECM. Topography and K+ flux images were obtained simultaneously at a polycarbonate membrane filter with 5 μm pores and human embryonic kidney 293 cells (HEK293). Several areas containing a K+ flux larger than the surrounding areas were found in the SECM images of the HEK293 cells, which indicated the existence of K+ channels. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032700
- Volume :
- 86
- Issue :
- 17
- Database :
- Academic Search Index
- Journal :
- Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 98521144
- Full Text :
- https://doi.org/10.1021/ac502444y