Back to Search
Start Over
Determination of autoinducer-2 in biological samples by high-performance liquid chromatography with fluorescence detection using pre-column derivatization.
- Source :
-
Journal of Chromatography A . Sep2014, Vol. 1361, p162-168. 7p. - Publication Year :
- 2014
-
Abstract
- Autoinducer-2 (AI-2), as a small-molecular-weight organic molecule secreted and perceived by various bacteria, enables intra- and inter-species communications. Quantitative determination of AI-2 is essential for exploring the bacterial AI-2-related physiological and biochemical processes. However, current strategies for sensitive detection of AI-2 require sophisticated instruments and complicated procedures. In this work, on the basis of the derivatization of AI-2 with 2,3-diaminonaphthalene, a simple, sensitive and cost-effective high-performance liquid chromatography with fluorescence detector (HPLC–FLD) method is developed for the quantitative detection of AI-2. Under the optimized conditions, this method had a broad linear range of 10–14,000 ng/ml ( R 2 = 0.9999), and a low detection limit of 1.0 ng/ml. Furthermore, the effectiveness of this approach was further validated through measuring the AI-2 concentrations in the cell-free culture supernatants of both Escherichia coli and Vibrio harveyi . [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00219673
- Volume :
- 1361
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatography A
- Publication Type :
- Academic Journal
- Accession number :
- 98145677
- Full Text :
- https://doi.org/10.1016/j.chroma.2014.07.103