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Changes and expressions of Redd1 in neurons and glial cells in the gerbil hippocampus proper following transient global cerebral ischemia.

Authors :
Choong Hyun Lee
Joon Ha Park
Jeong-Hwi Cho
Ji Hyeon Ahn
Bing Chun Yan
Jae-Chul Lee
Myoung Cheol Shin
Seung Hwan Cheon
Young Shin Cho
Jun Hwi Cho
Young-Guen Kwon
Dong-Keon Lee
Young-Myeong Kim
Moo-Ho Won
Source :
Journal of the Neurological Sciences. Sep2014, Vol. 344 Issue 1/2, p43-50. 8p.
Publication Year :
2014

Abstract

Redd1 (known as RTP801/Dig2/DDIT4) is a stress-induced protein, and it is known to be regulated in response to some stresses including hypoxia and oxidative stress. In the present study, we investigated the time-dependent changes in Redd1 immunoreactivity and its protein levels in the gerbil hippocampus proper (CA1-3 regions) after 5 min of transient global cerebral ischemia using immunohistochemistry and Western blot analysis. Redd1 immunoreactivity was apparently changed in the pyramidal neurons of the ischemic CA1 region, not in the pyramidal neurons of the ischemic CA2/3 region. Redd1 immunoreactivity in the CA1 pyramidal neurons was significantly increased at 6 h post-ischemia, decreased until 1 day post-ischemia, increased again at 2 days post-ischemia and weakly observed at 5 days post-ischemia. Especially, at 5 days after ischemic damage, Redd1 immunoreactivity was newly expressed in astrocytes and GABAergic interneurons in the CA1 region. Redd1 protein levels in the ischemic CA1 region were changed like the pattern of the Redd1 immunoreactivity. These results indicate that Redd1 immunoreactivity and protein levels are increased in the ischemic CA1 region at an early time after ischemic damage and that the increased Redd1 expression may be closely related to the delayed neuronal death of the CA1 pyramidal neurons following 5 min of transient global cerebral ischemia. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
0022510X
Volume :
344
Issue :
1/2
Database :
Academic Search Index
Journal :
Journal of the Neurological Sciences
Publication Type :
Academic Journal
Accession number :
97604827
Full Text :
https://doi.org/10.1016/j.jns.2014.06.016