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A comprehensive ligninolytic pre-treatment approach from lignocellulose green biotechnology to produce bio-ethanol.
- Source :
-
Chemical Engineering Research & Design: Transactions of the Institution of Chemical Engineers Part A . Aug2014, Vol. 92 Issue 8, p1571-1578. 8p. - Publication Year :
- 2014
-
Abstract
- In the present study, we developed a novel ligninolytic enzymes based pre-treatment method for lignocellulosic wheat straw to depolymerize lignin and expose the cellulose polymers to produce bio-ethanol. Wheat straw was pre-treated with ligninolytic enzymes extract produced from Ganoderma lucidum under optimum solid state fermentation conditions. The pre-treated biomass was further subjected to the enzymatic hydrolysis by the crude unprocessed cellulases (β-1,4 endoglucanase, 53.5 ± 1.24 U/mL; (β-1,4 exoglucanase, 41.3 ± 1.31 U/mL; (β-1,4 glucosidase, 46.8 ± 1.43 U/mL; and xylanase 39 ± 2.2 U/mL) produced by Trichoderma harzaianum. Under optimal conditions for enzymatic saccharification, 10% (w/v) of pre-treated biomass was hydrolyzed completely and converted to 72.5 and 2.4 g/L of glucose and xylose, respectively. Initial time screening Sequential Saccharification and Fermentation (SSF) of the concentrated enzymatic hydrolyzate (10%, w/v) by Saccharomyces cereuisiae produced 22.6 g/L ethanol in a fermented medium after 72 h of temperature controlled incubation at 37 °C. For maximum ethanol production, different physical and nutritional parameters like pH, temperature, substrate level and inoculum sizes were optimized. Under optimal conditions ethanol production of 33.5 g/L was obtained from ligninolytic treated residual (wheat straw) biomass. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02638762
- Volume :
- 92
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Chemical Engineering Research & Design: Transactions of the Institution of Chemical Engineers Part A
- Publication Type :
- Academic Journal
- Accession number :
- 97603378
- Full Text :
- https://doi.org/10.1016/j.cherd.2013.09.003