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Suppressive Effects of Britanin, a Sesquiterpene Compound Isolated from Inulae Flos, on Mast Cell-Mediated Inflammatory Responses.
- Source :
-
American Journal of Chinese Medicine . 2013, Vol. 42 Issue 4, p935-947. 13p. 2 Color Photographs, 1 Black and White Photograph, 2 Charts. - Publication Year :
- 2014
-
Abstract
- Mast cells are central players in immediate-type hypersensitvity and inflammatory responses. In the present study, the effects of britanin on the passive cutaneous anaphylaxis (PCA) reaction in mice and on the phorbol 12-myristate 13-acetate and calcium ionophore A23187 (PMACI)-induced production of pro-inflammatory cytokines in human mast cell line (HMC-1) were evaluated. The oral administration of britanin (10-20 mg/kg) decreased the mast cell-mediated PCA reaction in IgE-sensitized mice. In the activity and mechanism of britanin in vitro assay, britanin suppressed the gene expression and secretion of pro-inflammatory cytokines in a dose-dependent manner in HMC-1. In addition, britanin attenuated PMACI-induced activation of NF-κB as indicated by the inhibition of the degradation of IκBα, nuclear translocation of NF-κB, NF-κB/DNA binding activity assay, and blocked the phosphorylation of p38 MAP kinase, in a dose-dependent manner. We conclude that britanin may have potential as a treatment for allergic-inflammatory diseases. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ALLERGY drug therapy
*ANALYSIS of variance
*ANAPHYLAXIS
*ANIMAL experimentation
*ANTI-inflammatory agents
*CYTOKINES
*ENZYME-linked immunosorbent assay
*GENE expression
*HIGH performance liquid chromatography
*MAST cells
*MEDICINAL plants
*MICE
*POLYMERASE chain reaction
*RESEARCH funding
*TERPENES
*WESTERN immunoblotting
*PLANT extracts
*DATA analysis software
*IN vitro studies
*PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 0192415X
- Volume :
- 42
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- American Journal of Chinese Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 97469129
- Full Text :
- https://doi.org/10.1142/S0192415X14500591