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The pKa of the general acid/base carboxyl group of a glycosidase cycles during catalysis: A...
- Source :
-
Biochemistry . 8/06/96, Vol. 35 Issue 31, p9958. 9p. 8 Diagrams, 6 Graphs. - Publication Year :
- 1996
-
Abstract
- Determines the pKa of the general acid/base carboxyl group of a glycosidase cycles during catalysis by studying Bacillus circulans xylanase. Function of Glu78 as a nucleophile to form the intermediate; Function of Glu172 as a general acid catalyst during glycosylation, protonating the departing aglycone; Function as a general base during deglycosylation.
- Subjects :
- *GLYCOSIDASES
*BACILLUS (Bacteria)
Subjects
Details
- Language :
- English
- ISSN :
- 00062960
- Volume :
- 35
- Issue :
- 31
- Database :
- Academic Search Index
- Journal :
- Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 9703200401
- Full Text :
- https://doi.org/10.1021/bi9613234