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A sequential co-extraction method for DNA, RNA and protein recovery from soil for future system-based approaches.
- Source :
-
Journal of Microbiological Methods . Aug2014, Vol. 103, p118-123. 6p. - Publication Year :
- 2014
-
Abstract
- A co-extraction protocol that sequentially isolates core biopolymer fractions (DNA, RNA, protein) from edaphic microbial communities is presented. In order to confirm compatibility with downstream analyses, bacterial T-RFLP profiles were generated from the DNA- and RNA-derived fractions of an arid-based soil, with metaproteomics undertaken on the corresponding protein fraction. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01677012
- Volume :
- 103
- Database :
- Academic Search Index
- Journal :
- Journal of Microbiological Methods
- Publication Type :
- Academic Journal
- Accession number :
- 96931215
- Full Text :
- https://doi.org/10.1016/j.mimet.2014.06.004