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Effect of photodynamic therapy with hypocrellin B on apoptosis, adhesion, and migration of cancer cells.
- Source :
-
International Journal of Radiation Biology . Jul2014, Vol. 90 Issue 7, p575-579. 5p. - Publication Year :
- 2014
-
Abstract
- Purpose: In the present study, we investigated effects of photodynamic therapy with hypocrellin B on apoptosis, adhesion, and migration of cancer cells in vitro. Materials and methods: Human ovarian cancer HO-8910 cell as a cancer model cell was incubated with hypocrellin B at a concentration of 2.5 μM for 5 h and irradiated by light from a light-emitting diodes (LED) source. Cell apoptosis was analyzed by flow cytometry with annexin V/propidium iodide (PI) staining and nuclear staining 6 h after hypocrellin B photoirradiation. Cell adhesion was assessed using the 3-(4, 5-dimthylthiazol-2-yl)-2, 5 diphenyl-tetrazolium bromide (MTT) assay 4 h after photodynamic treatment. Cell migration was measured 48 h after photodynamic treatment. Results: Flow cytometry with annexin V/PI staining showed that early apoptotic and late apoptotic (necrotic) rates following photodynamic therapy with hypocrellin B markedly increased to 16.40% and 24.67%, respectively. Nuclear staining found nuclear condensation and typical apoptotic body in the treated cells. The number of cell migration was significantly decreased to 183 ± 28 after photodynamic therapy with hypocrellin B ( p < 0.01). Light irradiation alone and hypocrellin B alone had no significant effect on cell migration. The cell adhesion inhibitory rate due to photodynamic action of hypocrellin B was 53.2 ± 1.8%, significantly higher than 2.7 ± 2.1% of light treatment alone and 1.0 ± 0.4% of hypocrellin B treatment alone ( p < 0.01). Conclusion: The findings demonstrated that photodynamic therapy with hypocrellin B remarkably induced apoptosis and inhibited adhesion and migration of cancer cells in vitro. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PHOTODYNAMIC therapy
*OVARIAN cancer
*CANCER cells
*APOPTOSIS
*CELL adhesion
Subjects
Details
- Language :
- English
- ISSN :
- 09553002
- Volume :
- 90
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- International Journal of Radiation Biology
- Publication Type :
- Academic Journal
- Accession number :
- 96871254
- Full Text :
- https://doi.org/10.3109/09553002.2014.906765