Selection of the best sperm processing method in leukocytospermia.

Introduction: Leukocytospermia was seen in 10% of infertile men. Sperm DNA fragmentation has been proposed as a cause of male infertility. The processing of leukocytospermic semen leads to increase sperm DNA fragmentation and decrease of sperm quality and its fertilizing ability in infertile men. The aim of this study was the selection of the best sperm processing method for leukocytospermic semen to decrease DNA fragmentation. Materials and Methods: Semen samples with leukocytospermia were collected from 50 infertile men who referred to the Avicenna Infertility Center affiliated to Avicenna Research Institute. The samples have divided into 5 fractions for processing through the following methods; simple washing, Density gradient centrifugation (DGC), Glass wool, Swim up and neat semen. Sperm DNA fragmentation of prepared sperm was evaluated through SCD method by SDFA kit. Semen analysis was carried out according to WHO criteria. The results were evaluated by performing statistical analysis with statistical package of SPSS v21. Results: The results were showed that percentage of sperm with DNA fragmentation selected by Glass wool procedure (10.81±1.21) were significantly (p<0.05) lower than other methods in leukocytospermia samples in comparison with control group (23.98±1.54). Conclusion: According to this study the most appropriate method for separating the best sperm from leukocytospermic samples with minimum Sperm DNA fragmentation is Glass wool procedure. [ABSTRACT FROM AUTHOR]