Investigation of KDR gene polymorphisms (-604, 1719 and 1192) in women with unexplained recurrent pregnancy loss in comparison with normal women.

Introduction: Recurrent pregnancy loss is the most important fertility problem which affects about 1-5% of pregnant women. One of the possible genetic causes of unknown recurrent pregnancy loss is impairment in angiogenesis system and one of the most important genes is Kinas insert-Domain-Receptor (KDR). It is found on vasculogenic and angiogenic precursor cells in human placenta. Determining the association between single nucleotide polymorphisms (SNPS) (-604, 1719 and 1192) of KDR gene in women with unexplained Recurrent pregnancy loss in comparison with healthy women. Materials and Methods: In this study 110 women with history of recurrent pregnancy loss in the reproductive age as case and 120 healthy women with no history of abortion and at least one healthy child are chosen as control group. Genotyping of these polymorphisms were analyzed using the Amplification Refractory Mutation System-Polymerase Chain Reaction (ARMS-PCR) technique. Results: Genotype frequencies of SNP -604 in women with unexplained recurrent pregnancy loss were as follows TT (26.4%) TC (45.5%) and CC (28.2%) while in normal group genotypes were 30%, 43.4% and 26.7%respectively. Genotypes AA, AT and TT of SNP(1719) presented frequencies of 30%, 2.8%and 67.3% respectively in case group and 3.4%, 32.5% and 64.2% in controls. Distribution of Genotypes in SNP 1192 was 79.1%, 19% and 1.8% for GG, AG and AA in case and 80%, 20% and 0% in the same order in controls . Conclusion: Results of this study demonstrated no association between recurrent pregnancy loss and -604, 1719 and 1192 SNPS of KDR genes. [ABSTRACT FROM AUTHOR]