Comparative method validation for closantel determination in cattle and sheep milk according to European Union Volume 8 and Veterinary International Conference on Harmonization guidelines.

Highlights: [•] LC–MS/MS method for quantitative determination of closantel in bovine and ovine milk. [•] Optimized extraction and clean-up using Oasis® MAX® SPE columns. [•] Method validation of bovine milk according to EU Volume 8 (2005). [•] Method validation of ovine milk according to EU Volume 8 (2005) and VICH GL49 (2012). [•] Although both guidelines cover similar parameters, significant differences exist. [ABSTRACT FROM AUTHOR]