Isolation and screened neuroprotective active constituents from the roots and rhizomes of Valeriana amurensis.

Abstract: In previous study, we have screened the effective fraction against Alzheimer’s disease (AD-EF) from the extracts of roots and rhizomes of Valeriana amurensis, based on which neuroprotective active constituents from AD-EF were investigated. Six new compounds 1–6, including four iridoids (xiecaoside A–C and xiecaoline A), one pinane-type monoterpeneglucoside (xiecaoside D), and one phenylpropanoid glycoside (xiecaoside E) were isolated together with 11 known compounds 7–17. The structures of 1–6 were elucidated by their spectroscopic data. The protective effects of compounds 1–17 on PC12 cells with neurotoxicity induced by amyloid-beta 1–42 (A?1–42) was also investigated, respectively. Consequently, compound 6 and lignans 11–17 were responsible for protecting against A?-induced toxicity in PC12 cells. [Copyright &y& Elsevier]