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A detailed three-step protocol for live imaging of intracellular traffic in polarized primary porcine RPE monolayers.
- Source :
-
Experimental Eye Research . Jul2014, Vol. 124, p74-85. 12p. - Publication Year :
- 2014
-
Abstract
- Highlights: [•] We present a detailed 3-step protocol for live imaging of polarized primary RPE. [•] Step 1: a protocol for primary porcine retinal pigment epithelial culture. [•] This method results in highly polarized monolayers within 7 days. [•] Step 2: two gene delivery protocols that yield efficient transgene expression. [•] Step 3: High speed live imaging to visualize intracellular traffic in the RPE. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00144835
- Volume :
- 124
- Database :
- Academic Search Index
- Journal :
- Experimental Eye Research
- Publication Type :
- Academic Journal
- Accession number :
- 96582635
- Full Text :
- https://doi.org/10.1016/j.exer.2014.05.003