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Chromosomal aneuploidy affects the global proteome equilibrium of colorectal cancer cells.
- Source :
-
Analytical Cellular Pathology: Cellular Oncology . 2013, Vol. 36 Issue 5, p149-161. 13p. - Publication Year :
- 2013
-
Abstract
- BACKGROUND: Chromosomal aneuploidy has been identified as a prognostic factor in the majority of sporadic carcinomas. However, it is not known how chromosomal aneuploidy affects chromosome-specific protein expression in particular, and the cellular proteome equilibrium in general. OBJECTIVE: The aim was to detect chromosomal aneuploidy-associated expression changes in cell clones carrying trisomies found in colorectal cancer. METHODS: We used microcell-mediated chromosomal transfer to generate three artificial trisomic cell clones of the karyotypically stable, diploid, yet mismatch-deficient, colorectal cancer cell line DLD1 - each of them harboring one extra copy of either chromosome 3, 7 or 13. Protein expression differences were assessed by two-dimensional gel electrophoresis and mass spectrometry, compared to whole-genome gene expression data, and evaluated by PANTHER classification system and Ingenuity Pathway Analysis (IPA). RESULTS: In total, 79 differentially expressed proteins were identified between the trisomic clones and the parental cell line. Up-regulation of PCNA and HMGB1 as well as down-regulation of IDH3A and PSMB3 were revealed as trisomy-associated alterations involved in regulating genome stability. CONCLUSIONS: These results show that trisomies affect the expression of genes and proteins that are not necessarily located on the trisomic chromosome, but reflect a pathway-related alteration of the cellular equilibrium. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ANEUPLOIDY
*PROTEOMICS
*COLON cancer
*CANCER cells
*PROTEIN expression
Subjects
Details
- Language :
- English
- ISSN :
- 22107177
- Volume :
- 36
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Analytical Cellular Pathology: Cellular Oncology
- Publication Type :
- Academic Journal
- Accession number :
- 96445367
- Full Text :
- https://doi.org/10.1155/2013/249054