Integrating multiple bioassays to detect and assess impacts of sublethal exposure to metal mixtures in an estuarine fish.

Highlights: [•] We use fish development, morphology, behaviour and otoliths to assess metal toxicity. [•] Fish embryos exposed to metal mixtures had lower survival and delayed development. [•] This resulted in poorer quality larvae that exhibited weaker phototactic behaviour. [•] Differences in metal exposure levels were permanently recorded in embryonic otoliths. [•] Otoliths could be a useful tool for linking past exposure to poor quality phenotypes. [Copyright &y& Elsevier]